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Does this audio track create a bond between the brain and the central nervous system whilst stimulating the pineal gland?

The brain is part of the CNS so it is nonsensical to write of creating a bond between them.

The pineal gland's activity is primarily affected by light not by sound.

Is this just a hoax?

There is no Jeffery Marlon Harris FRCS registered as a Fellow at the Royal College of Surgeons

There is no county of Pembridgeshire in England or in the UK

There is no University of Pembridgeshire in England (as Oddthinking observed in a comment to the question)

The pineal gland produces melatonin. If you want extra melatonin in your bloodstream, you can eat plants that contain it, such as cherries. There is no need to use roundabout methods to stimulate your pineal gland.

Any video containing these falsehoods has no credibility.

Do humans have a third eye?

The "third eye" is a somewhat mystical name given to the pineal gland by somewhat mystical people. It's activity is affected by light levels which are sensed via photoreceptors in your eyes (the real ones). It doesn't provide any sensory capability itself (in the normally accepted meaning of "sense"). I could equally assert that humans have a second nose if I chose that as an alternative name for the appendix. Calling something an eye doesn't make it in any way like the two eyes in the front of your head. So yes humans have a "third eye" if you accept that name.